Mechanism of TCD (Thermal Conductivity Detector) in GC:
Gas chromatography (GC) is an analytical technique used to separate and analyze the individual components of a complex mixture. GC works by injecting a sample into a chromatographic column, which separates the components based on their unique chemical properties, such as boiling point or polarity. As the components elute from the column, they pass through a detector, which measures their concentration and generates a chromatogram, a graphical representation of the separation.
One type of detector commonly used in GC is the thermal conductivity
detector (TCD). TCD is a universal detector that can detect all components,
regardless of their chemical properties. TCD works by measuring the thermal
conductivity of the effluent gas stream from the column. The TCD consists of
two wire filaments, one a reference filament and the other a sample filament.
These filaments are heated to a constant temperature using a Wheatstone bridge
circuit.
The change in resistance of the sample filament is detected by the Wheatstone bridge circuit, which generates a voltage output that is proportional to the difference in resistance between the two filaments. This voltage output is then amplified and recorded as a peak on the chromatogram. The magnitude of the peak is proportional to the concentration of the component in the sample.
The TCD has several advantages over other GC detectors. It is a universal detector that can detect all components, regardless of their chemical properties. It is also highly sensitive and has a wide dynamic range, making it suitable for analyzing both trace and high-concentration samples. Additionally, TCD is a non-destructive detector, which means that the sample can be recovered after analysis for further testing.
However, TCD also has some limitations. It is not as selective as other detectors, such as mass spectrometry, and may not be able to distinguish between components with similar thermal conductivity. Additionally, TCD may be affected by changes in carrier gas flow rate, pressure, or composition, which can cause fluctuations in the baseline and affect the accuracy of the results.
In summary, the TCD detector in GC works by measuring the thermal conductivity of the effluent gas stream from the column using two wire filaments. The change in resistance of the sample filament is detected by a Wheatstone bridge circuit, which generates a voltage output that is proportional to the difference in resistance between the two filaments. This voltage output is then amplified and recorded as a peak on the chromatogram, with the magnitude of the peak proportional to the concentration of the component in the sample. While TCD has several advantages, it also has some limitations that should be considered when selecting a detector for GC analysis.
Abhishek Singh is the author of Pharma technical support. He is pharmaceutical professionals, having experience of more than 12 years in Quality.